1 PCAngsd Selection Scan

  • Genome-wide Fst scan between years of PWS pop using PCAngsd

1.1 Run PCAngsd selection on Pairwise comparison

  • Can be run for multiple populations
python /home/jamcgirr/apps/pcangsd/pcangsd.py -beagle /home/ktist/ph/data/new_vcf/MD7000/beagle/subpop/PWS91.PWS96_maf05_BEAGLE.PL.gz -o /home/ktist/ph/data/angsd/selection/PWS91.PWS96_selection -selection -sites_save 

# scripts to run at Fram: pcangsd_selection.sh 
# run pcansgd_selectionPWS.sh for aggregated PWS pop over years

1.2 Results

cols<-c("#0072b2","#cc79a7","#009e73","#d55e00","#56b4e9","#e69f00","#f0e442")

pop_info<-read.csv("../Data/Sample_metadata_892pops.csv")
pop_info<-pop_info[,c("Sample","pop","Year.Collected")]
colnames(pop_info)[3]<-"year"

#Based on the tutorial http://www.popgen.dk/software/index.php/PCAngsdTutorial

## function for QQplot
qqchi<-function(x,...){
    lambda<-round(median(x)/qchisq(0.5,1),2)
    qqplot(qchisq((1:length(x)-0.5)/(length(x)),1),x,ylab="Observed",xlab="Expected",...);abline(0,1,col=2,lwd=2)
    legend("topleft",paste("lambda=",lambda))
}

########  PWS pairwise run ###
pwapops<-c("PWS91" ,"PWS96","PWS07", "PWS17")
comb<-combn(pwapops, 2)
comb<-t(comb)
comb1<-comb[c(1,4,6,3),]

for (i in 1:nrow(comb1)){
    pop1<-comb1[i,1]
    pop2<-comb1[i,2]
    
    #s<-npyLoad(paste0("Data/PCAangsd/selection/",pop1,".",pop2,"_selection.selection.npy"))
    s<-npyLoad(paste0("../Data/PCAangsd/selection/pruned_",pop1,".",pop2,"_selection.selection.npy"))
    
    #how many PC axes were evaluated?
    nc<-ncol(s)
    
    ## make QQ plot to QC the test statistics
    #qqchi(s)
    
    # convert test statistic to p-value
    if (nc==1) pval<-1-pchisq(s,1)
    if (nc>1) {
        p$pval1<-pval[,1]
        p$pval2<-pval[,2]
        p$loc<-1:nrow(p)
        p$pval1.log<--log10(p$pval1)
        p$pval2.log<--log10(p$pval2)
        }
    
    #read the position info    
    p<-read.table(paste0("../Data/PCAangsd/selection/pruned_",pop1,".",pop2,"_selection.sites"),colC=c("factor","integer"),sep=":")
    names(p)<-c("chr","pos")
    
    ## make manhatten plot
    p$pval<-pval
    p$loc<-1:nrow(p)
    p$pval.log<--log10(p$pval)
    
    write.csv(p, paste0("../Output/PCA/selection/Fst_pval_Pruned", pop1,".",pop2,".csv"))
    write.csv(p[p$pval.log>4,], paste0("../Output/PCA/selection/Selected_highP_sites_Pruned", pop1,".",pop2,".csv"))
    
    ch<-unique(p$chr)
    
    #count the number of sites per chromosomes
    poss<-data.frame(chr=ch)
    k=1
    for (i in 1:nrow(poss)){
        df<-p[p$chr==poss$chr[i],]
        poss$start[i]<-k
        poss$end[i]<-k+nrow(df)-1
        k=k+nrow(df)
    }
    
    poss$x<-poss$start+(poss$end-poss$start)/2
    
    #color vectors
    colors<-rep(c("steelblue","lightblue"), times=13)
    p$chr<-factor(p$chr, levels=poss$chr)
    ggplot(data=p, aes(x=loc, y=pval.log, color=chr))+
        geom_point(size=0.15)+
        scale_color_manual(values=colors, guide='none')+
        scale_x_continuous(name="Chromosome", breaks=poss$x, labels=gsub("chr",'',poss$chr))+
        theme_classic()+ylab("-log10(p-value)")+
        ggtitle(paste0(pop1," vs. ",pop2))
    ggsave(paste0("../Output/PCA/selection/pcangsd_selection_",pop1,"_",pop2,"_plot.png"), width = 6, height = 3, dpi = 300)    
}


1.3 PCAnsgd for all years of PWS

## PWS together
s<-npyLoad("../Data/PCAangsd/selection/PWS_selection.selection.npy")

## make QQ plot to QC the test statistics
#qqchi(s)
# convert test statistic to p-value
pval<-1-pchisq(s,1)

## read positions 
p<-read.table("../Data/PCAangsd/selection/PWS_selection.sites",colC=c("factor","integer"),sep=":")
names(p)<-c("chr","pos")
p$pval1<-pval[,1]
p$loc<-1:nrow(p)
p$pval1.log<--log10(p$pval1)

#count the number of sites per chromosomes
ch<-unique(p$chr)
poss<-data.frame(chr=ch)
k=1
for (i in 1:nrow(poss)){
    df<-p[p$chr==poss$chr[i],]
    poss$start[i]<-k
    poss$end[i]<-k+nrow(df)-1
    k=k+nrow(df)
}

poss$x<-poss$start+(poss$end-poss$start)/2

colors<-rep(c("steelblue","lightblue"), times=13)
p$chr<-factor(p$chr, levels=poss$chr)

ggplot(data=p, aes(x=loc, y=pval1.log, color=chr))+
    geom_point(size=0.1)+
    scale_color_manual(values=colors, guide='none')+
    scale_x_continuous(name="Chromosome position", breaks=poss$x, labels=gsub("chr",'',poss$chr))+
    theme_classic()+ylab("-log10(p-value)")+
    ggtitle("PWS")
ggsave("../Output/PCA/selection/PWS_pcangsd_selection.png", width = 12, height = 6, dpi=300)    

1.4 High P-value loci from each pop pair

#for each population pair
pwapops<-c("PWS91" ,"PWS96","PWS07", "PWS17")
comb<-combn(pwapops, 2)
comb<-t(comb)
comb1<-comb[c(1,4,6,3),]

#create windows to be assigned for each site
chr <- read.table('../Data/new_vcf/chr_sizes.bed')
chr<-chr[,-2]
colnames(chr)<-c("chr", "len")
#chrmax$start<-c(0,cumsum(chrmax$len)[1:(nrow(chrmax)-1)])
#setkey(chrmax, chr)

winsz <- 50000 # window size
# use seq to find the start points of each window
windows<-data.frame()
for (i in 1:nrow(chr)){
    ch<-chr$len[chr$chr==paste0("chr",i)]
    window_start <- seq(from = 1, to = ch-50000, by = winsz)
    window_stop <- window_start + (winsz-1)
    win <- data.frame(start = window_start, stop = window_stop, 
                      mid = window_start + ((window_stop-window_start)/2-0.5))
    win$chr<-paste0("chr",i)
    win$ch<-i
    windows<-rbind(windows, win)
}
setDT(windows)
fstdat<-list()
Fst<-data.frame()
for (i in 1: nrow(comb1)){
    pop1<-comb1[i,1]
    pop2<-comb1[i,2]
    df<-fread(paste0("../Output/PCA/selection/Selected_highP_sites_Pruned", pop1,".",pop2,".csv"))
    df<-df[,-1]
    #assign the window info
    df<-windows[df, .(chr, pos, pval, pval.log, start, stop, mid), on=.(chr=chr, start <=pos, stop>=pos)]
    #create a unique id for the window
    df$win_id<-paste0(df$chr,"_",df$mid)
    df$pops<-paste0(pop1,".",pop2)
    fstdat[[i]]<-df
    names(fstdat)[i]<-paste0(pop1,".",pop2)
    Fst<-rbind(Fst, df)
}

#Find overlapping positions among pairs?
lapply(fstdat, "[[", "win_id")

Reduce(intersect, list(fstdat[[1]]$win_id , fstdat[[2]]$win_id))
Reduce(intersect, list(fstdat[[2]]$win_id , fstdat[[3]]$win_id))
Reduce(intersect, list(fstdat[[3]]$win_id , fstdat[[4]]$win_id))

Fst$chr<-factor(Fst$chr, levels=paste0("chr",1:26))
ggplot(Fst, aes(x=pos, y=pval.log, color=pops))+
    geom_point()+
    facet_wrap(~chr)+
   theme_bw()+xlab("Genome position")+
    ylab("-log10(P-val)")+theme(legend.title = element_blank(), axis.text.x = element_blank())
ggsave("../Output/Fst/pcangsd_scan_highPsites.png", width=9, height=6, dpi=300)



---
title: "Selection scan (Fst outliers) between years"
output:
  html_notebook:
      toc: true 
      toc_float: true
      number_sections: true
      theme: lumen
      highlight: tango
      code_folding: hide
      df_print: paged
---
```{r eval=FALSE, message=FALSE, warning=FALSE, include=FALSE}
source("../Rscripts/BaseScripts.R")
library(stringr)
library(gridExtra)
library(RcppCNPy)

```

# PCAngsd Selection Scan
* Genome-wide Fst scan between years of PWS pop using PCAngsd   

## Run PCAngsd selection on Pairwise comparison
* Can be run for multiple populations  

```{bash}
python /home/jamcgirr/apps/pcangsd/pcangsd.py -beagle /home/ktist/ph/data/new_vcf/MD7000/beagle/subpop/PWS91.PWS96_maf05_BEAGLE.PL.gz -o /home/ktist/ph/data/angsd/selection/PWS91.PWS96_selection -selection -sites_save 

# scripts to run at Fram: pcangsd_selection.sh 
# run pcansgd_selectionPWS.sh for aggregated PWS pop over years

```

## Results

```{r eval=FALSE, message=FALSE, warning=FALSE}
cols<-c("#0072b2","#cc79a7","#009e73","#d55e00","#56b4e9","#e69f00","#f0e442")

pop_info<-read.csv("../Data/Sample_metadata_892pops.csv")
pop_info<-pop_info[,c("Sample","pop","Year.Collected")]
colnames(pop_info)[3]<-"year"

#Based on the tutorial http://www.popgen.dk/software/index.php/PCAngsdTutorial

## function for QQplot
qqchi<-function(x,...){
    lambda<-round(median(x)/qchisq(0.5,1),2)
    qqplot(qchisq((1:length(x)-0.5)/(length(x)),1),x,ylab="Observed",xlab="Expected",...);abline(0,1,col=2,lwd=2)
    legend("topleft",paste("lambda=",lambda))
}

########  PWS pairwise run ###
pwapops<-c("PWS91" ,"PWS96","PWS07", "PWS17")
comb<-combn(pwapops, 2)
comb<-t(comb)
comb1<-comb[c(1,4,6,3),]

for (i in 1:nrow(comb1)){
    pop1<-comb1[i,1]
    pop2<-comb1[i,2]
    
    #s<-npyLoad(paste0("Data/PCAangsd/selection/",pop1,".",pop2,"_selection.selection.npy"))
    s<-npyLoad(paste0("../Data/PCAangsd/selection/pruned_",pop1,".",pop2,"_selection.selection.npy"))
    
    #how many PC axes were evaluated?
    nc<-ncol(s)
    
    ## make QQ plot to QC the test statistics
    #qqchi(s)
    
    # convert test statistic to p-value
    if (nc==1) pval<-1-pchisq(s,1)
    if (nc>1) {
        p$pval1<-pval[,1]
        p$pval2<-pval[,2]
        p$loc<-1:nrow(p)
        p$pval1.log<--log10(p$pval1)
        p$pval2.log<--log10(p$pval2)
        }
    
    #read the position info    
    p<-read.table(paste0("../Data/PCAangsd/selection/pruned_",pop1,".",pop2,"_selection.sites"),colC=c("factor","integer"),sep=":")
    names(p)<-c("chr","pos")
    
    ## make manhatten plot
    p$pval<-pval
    p$loc<-1:nrow(p)
    p$pval.log<--log10(p$pval)
    
    write.csv(p, paste0("../Output/PCA/selection/Fst_pval_Pruned", pop1,".",pop2,".csv"))
    write.csv(p[p$pval.log>4,], paste0("../Output/PCA/selection/Selected_highP_sites_Pruned", pop1,".",pop2,".csv"))
    
    ch<-unique(p$chr)
    
    #count the number of sites per chromosomes
    poss<-data.frame(chr=ch)
    k=1
    for (i in 1:nrow(poss)){
        df<-p[p$chr==poss$chr[i],]
        poss$start[i]<-k
        poss$end[i]<-k+nrow(df)-1
        k=k+nrow(df)
    }
    
    poss$x<-poss$start+(poss$end-poss$start)/2
    
    #color vectors
    colors<-rep(c("steelblue","lightblue"), times=13)
    p$chr<-factor(p$chr, levels=poss$chr)
    ggplot(data=p, aes(x=loc, y=pval.log, color=chr))+
        geom_point(size=0.15)+
        scale_color_manual(values=colors, guide='none')+
        scale_x_continuous(name="Chromosome", breaks=poss$x, labels=gsub("chr",'',poss$chr))+
        theme_classic()+ylab("-log10(p-value)")+
        ggtitle(paste0(pop1," vs. ",pop2))
    ggsave(paste0("../Output/PCA/selection/pcangsd_selection_",pop1,"_",pop2,"_plot.png"), width = 6, height = 3, dpi = 300)    
}

```
![](../Output/PCA/selection/pcangsd_selection_PWS91_PWS96_plot.png)
![](../Output/PCA/selection/pcangsd_selection_PWS96_PWS07_plot.png)  
![](../Output/PCA/selection/pcangsd_selection_PWS07_PWS17_plot.png)
![](../Output/PCA/selection/pcangsd_selection_PWS91_PWS17_plot.png)

## PCAnsgd for all years of PWS  
```{r eval=FALSE, message=FALSE, warning=FALSE}
## PWS together
s<-npyLoad("../Data/PCAangsd/selection/PWS_selection.selection.npy")

## make QQ plot to QC the test statistics
#qqchi(s)
# convert test statistic to p-value
pval<-1-pchisq(s,1)

## read positions 
p<-read.table("../Data/PCAangsd/selection/PWS_selection.sites",colC=c("factor","integer"),sep=":")
names(p)<-c("chr","pos")
p$pval1<-pval[,1]
p$loc<-1:nrow(p)
p$pval1.log<--log10(p$pval1)

#count the number of sites per chromosomes
ch<-unique(p$chr)
poss<-data.frame(chr=ch)
k=1
for (i in 1:nrow(poss)){
    df<-p[p$chr==poss$chr[i],]
    poss$start[i]<-k
    poss$end[i]<-k+nrow(df)-1
    k=k+nrow(df)
}

poss$x<-poss$start+(poss$end-poss$start)/2

colors<-rep(c("steelblue","lightblue"), times=13)
p$chr<-factor(p$chr, levels=poss$chr)

ggplot(data=p, aes(x=loc, y=pval1.log, color=chr))+
    geom_point(size=0.1)+
    scale_color_manual(values=colors, guide='none')+
    scale_x_continuous(name="Chromosome position", breaks=poss$x, labels=gsub("chr",'',poss$chr))+
    theme_classic()+ylab("-log10(p-value)")+
    ggtitle("PWS")
ggsave("../Output/PCA/selection/PWS_pcangsd_selection.png", width = 12, height = 6, dpi=300)    
```
![](../Output/PCA/selection/PWS_pcangsd_selection.png)

## High P-value loci from each pop pair

```{r echo=TRUE, message=FALSE, warning=FALSE}
#for each population pair
pwapops<-c("PWS91" ,"PWS96","PWS07", "PWS17")
comb<-combn(pwapops, 2)
comb<-t(comb)
comb1<-comb[c(1,4,6,3),]

#create windows to be assigned for each site
chr <- read.table('../Data/new_vcf/chr_sizes.bed')
chr<-chr[,-2]
colnames(chr)<-c("chr", "len")
#chrmax$start<-c(0,cumsum(chrmax$len)[1:(nrow(chrmax)-1)])
#setkey(chrmax, chr)

winsz <- 50000 # window size
# use seq to find the start points of each window
windows<-data.frame()
for (i in 1:nrow(chr)){
    ch<-chr$len[chr$chr==paste0("chr",i)]
    window_start <- seq(from = 1, to = ch-50000, by = winsz)
    window_stop <- window_start + (winsz-1)
    win <- data.frame(start = window_start, stop = window_stop, 
                      mid = window_start + ((window_stop-window_start)/2-0.5))
    win$chr<-paste0("chr",i)
    win$ch<-i
    windows<-rbind(windows, win)
}
setDT(windows)
fstdat<-list()
Fst<-data.frame()
for (i in 1: nrow(comb1)){
    pop1<-comb1[i,1]
    pop2<-comb1[i,2]
    df<-fread(paste0("../Output/PCA/selection/Selected_highP_sites_Pruned", pop1,".",pop2,".csv"))
    df<-df[,-1]
    #assign the window info
    df<-windows[df, .(chr, pos, pval, pval.log, start, stop, mid), on=.(chr=chr, start <=pos, stop>=pos)]
    #create a unique id for the window
    df$win_id<-paste0(df$chr,"_",df$mid)
    df$pops<-paste0(pop1,".",pop2)
    fstdat[[i]]<-df
    names(fstdat)[i]<-paste0(pop1,".",pop2)
    Fst<-rbind(Fst, df)
}

#Find overlapping positions among pairs?
lapply(fstdat, "[[", "win_id")

Reduce(intersect, list(fstdat[[1]]$win_id , fstdat[[2]]$win_id))
Reduce(intersect, list(fstdat[[2]]$win_id , fstdat[[3]]$win_id))
Reduce(intersect, list(fstdat[[3]]$win_id , fstdat[[4]]$win_id))

Fst$chr<-factor(Fst$chr, levels=paste0("chr",1:26))
ggplot(Fst, aes(x=pos, y=pval.log, color=pops))+
    geom_point()+
    facet_wrap(~chr)+
   theme_bw()+xlab("Genome position")+
    ylab("-log10(P-val)")+theme(legend.title = element_blank(), axis.text.x = element_blank())
ggsave("../Output/Fst/pcangsd_scan_highPsites.png", width=9, height=6, dpi=300)
```
![](../Output/Fst/pcangsd_scan_highPsites.png)



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